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centrifuge for 5 minutes to discard the supernatant
note: the environment temperature and reagent temperature will affect the time needed for dyeing
Dilution Buffer （1×） Can be used as a standard curve zero (0 pg/mL）。
Mainly used for leukemia immunophenotyping
Reference 1、Coquet J M
Mouse IL-1β ELISA Kit Size:96T centrifuge for 5 minutes toProduct Specification Theory Double antibody sandwich enzyme linked immunosorbent assay was used in this kit. Specific anti mouse IL 1 antibody was precoated on a high affinity microplate. The standard substance, the test sample and the biotinylated detection antibody were added into the microplate Wells. After incubation, the IL 1 present in the sample combined with the solid phase antibody and the detection antibody to form immune complexes. After