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Serum: Whole blood samples collected in serum separation tubes were placed at room temperature 2 Hour or 2-8℃ Overnight
It is necessary to conduct pre-experiments similar to cell number gradient detection
Expression System HEK293 Molecular Weight 30-40kDa (Reducing) Purity > 95% by SDS-PAGE & SEC-HPLC Endotoxin <1EU/μg Conjugation Biotin Tag Avi Tag
Rotamase A
and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Plasminogen Activator and Urokinase (uPA)
UniOne® TR-FRET Human IL21/IL21R Binding Kit Size:2500T Serum: Whole blood samples collectedProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the interaction between IL21 and IL21R. Thismethod enables simple and rapid high throughput screening of inhibitors andantibody blockers. As illustrated, the interaction between IL21 and IL21R isdetected using an Eu labeled anti Tag1 antibody (TR FRET donor) and anAcceptor labeled anti Tag2 antibody
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