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(1) The normal colon tissue of mice after collection must be placed in a sampling bottle of pre-cooled (2-8 °C) tissue preservation solution E
(7) 200 μL of Matrigel/cell mixture was seeded into a 24-well plate at 30 μL per well
The Matrigel is maintained at 0-4 ℃ throughout the process。 Place the cell culture plate in a 37 ℃ incubator for 10-15 minutes
application dilution species Sandwich ELISA N/A CLIA N/A CLIA N/A N/A
sealed (dry at low temperature) and stored
Human FGF-9 ELISA Kit Size:96T (1) The normal colon tissueProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
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