Setting of positive control:
Spike protein is a large type I transmembrane protein containing two subunits S1 and S2
EGFR is overexpressed or mutated in many cancers
and it is also essential for the function of follicular helper T cells
DNase I (2 U/μL) was diluted 1: 100 with 1 × DNase I Buffer to a final concentration of 20 U/mL of the working solution
E-Cadherin Recombinant Rabbit mAb (HRP Conjugate) (S-438-5) Functional Antibody Setting of positive control:Product Specification Host Rabbit Antigen E Cadherin Synonyms Cadherin 1, CAM 120 80, Epithelial cadherin, Uvomorulin, CD324, Uvomorulin, L CAM Immunogen Recombinant Protein Location Cell membrane Accession P12830 Clone Number S 438 5 Antibody Type Recombinant mAb Isotype IgG Application WB Reactivity Hu Purification Protein A Concentration 2 mg ml Conjugation HRP Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05%BSA, 0. 03% Proclin